Four-week-old WT mice were fed a GuD for four weeks and then divided into four groups (n = 4–6 per group). The rIL-18 intervention procedure was adopted from the studies by Seregin SS et al.76 (link) and Levy M et al.22 (link) From days 29 to 31, two groups received either recombinant mouse interleukin-18 (rIL-18, 1 µg/mouse; BioLegend), and another two groups received vehicle (veh.) intraperitoneally (i.p.). After 2 days of last injection, experimental groups were either switched to DSS (1.4% w/v) containing water (GuD+ veh. + DSS and GuD+ rIL-18+ DSS) or continued on regular water (GuD+ veh.+ water and GuD+ rIL-18+ water). After 7 days of DSS administration, mice were euthanized to collect samples for analyzing colitis markers. Mice were maintained on the GuD throughout the study.
Protocol detail
Dietary Modulation of Colitis Progression
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Corresponding Organization : Pennsylvania State University
Other organizations : Icahn School of Medicine at Mount Sinai, Inserm, Institut Pasteur, Université Paris Cité, Centre National de la Recherche Scientifique
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Variable analysis
independent variables
- Diet (GuD)
- RIL-18 treatment (rIL-18 vs. vehicle)
- Colitis markers
- WT mice
- Age of mice (four-week-old)
- Duration of diet and treatments (four weeks)
- DSS administration (1.4% w/v, 7 days)
- Positive control: GuD+ rIL-18+ water group
- Negative control: GuD+ veh.+ water group
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