LDL Aggregation by Sphingomyelinase
Corresponding Organization : University of Reading
Variable analysis
- Incubation of native LDL (2 mg protein/mL) with sphingomyelinase (Bacillus cereus, catalogue number S9396-25UN, Sigma) at 10 mU/mL
- Attenuance (absorbance plus light scattering) at 680 nm
- Native LDL (2 mg protein/mL)
- Phosphate buffer containing EDTA
- Sterilisation by membrane filtration (0.45 μm)
- Positive control: Not explicitly mentioned
- Negative control: Not explicitly mentioned
Annotations
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