MALDI-TOF Sample Preparation and Analysis

Samples for MALDI-TOF mass spectrometric analysis were prepared using HyperSep-C18-tips (Thermo Scientific, Schwerte, Germany) according to the manufacturer’s recommendations. Sample application was performed via dried-droplet on MSP 96 target ground steel (Bruker Daltonics, Bremen, Germany) with 1 µl sinapinic-matrix (45 mmol/l sinapinic acid in 70% (v/v) H₂O, 30% (v/v) acetonitrile, 0.3% (v/v) trifluoroacetic acid). After drying under vacuum, samples were analyzed using the LP-mode and 60–90% laser intensity by a Microflex device (Bruker Daltonics, Bremen, Germany). The free software mMass v. 5.5.0 was used to evaluate the mass spectra^{73 (link)}.

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Wüstenhagen D.A., Lukas P., Müller C., Aubele S.A., Hildebrandt J.P, & Kubick S. (2020). Cell-free synthesis of the hirudin variant 1 of the blood-sucking leech Hirudo medicinalis. Scientific Reports, 10, 19818.

Publication 2020

MSP 96 target ground steel Microflex device

Acetonitrile Device Maldi Mass spectrometric Sinapinic Sinapinic acid Steel Trifluoroacetic acid Vacuum

Corresponding Organization : University of Potsdam

Other organizations : Fraunhofer Institute for Cell Therapy and Immunology, Universität Greifswald

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Variable analysis

independent variables

Sample application technique (dried-droplet)

dependent variables

Mass spectra of samples

control variables

Samples prepared using HyperSep-C18-tips according to manufacturer's recommendations
Application of 1 µl sinapinic-matrix (45 mmol/l sinapinic acid in 70% (v/v) H2O, 30% (v/v) acetonitrile, 0.3% (v/v) trifluoroacetic acid)
Analysis using LP-mode and 60–90% laser intensity by a Microflex device
Mass spectra evaluated using the free software mMass v. 5.5.0

controls

No positive or negative controls were explicitly mentioned in the given information.

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