Deficient Mice for Immune Research

Ifnar1-, Stat1-, and Stat2-deficient mice were obtained from The Jackson Laboratory (Bar Harbor, ME). C57BL/6J mice were purchased from CLEA Japan (Tokyo, Japan). The anti-TNP mouse IgE (IGELb4) was described previously [30 (link)]. TNP-conjugated BSA (LGC Biosearch Technologies, Middlesex, UK, T5050-10) and 4-methylumbellyferyl-N-acetyl-β-glucosaminide (4-MUAG) (Sigma, St. Louis, MO, M2133), recombinant mouse IL-3 and mouse SCF (Peprotech, Rockey Hill, NJ, 213–13 and 250–03, respectively), recombinant mouse Thrombopoietin (Wako Pure Chemicals, Osaka, Japan, 202–19611), recombinant mouse IFN-α (Miltenyi Biotec 130-093-131), and the STAT3 inhibitor S3I-201 (Merck Millipore, Burlington, MA; hereafter MM, 573102) were purchased.