C. elegans HR-MAS NMR spectroscopy
was performed as previously described by Blaise et al.9 (link),10 (link) Spectra were reduced over the chemical range of 0.55–8.75
ppm to 8200 bins (10–3 ppm wide) with integration
of signal intensity. The residual water signal (δ = 4.5–5
ppm), residual methanol signal resulting from the formaldehyde fixation
step (δ = 3.32–3.39 ppm), and a noise area (δ =
5.5–6.5 ppm) were discarded prior to analysis. Spectra were
normalized using the probabilistic quotient normalization approach14 (link) with a median of all spectra as a reference
spectrum. We applied Pareto scaling on the data set for multivariate
analysis only.
Metabolite assignment was completed exploiting
reference data from the literature,9 (link),25 (link) the HMDB,15 (link) MMCD,16 (link) bbiorefcode-2-0-0
(Bruker, GmbH, Rheinstetten, Germany), and Chenomx NMR Suite 7.0 (Chenomx
Inc., Edmonton, Canada) spectral databases.
was performed as previously described by Blaise et al.9 (link),10 (link) Spectra were reduced over the chemical range of 0.55–8.75
ppm to 8200 bins (10–3 ppm wide) with integration
of signal intensity. The residual water signal (δ = 4.5–5
ppm), residual methanol signal resulting from the formaldehyde fixation
step (δ = 3.32–3.39 ppm), and a noise area (δ =
5.5–6.5 ppm) were discarded prior to analysis. Spectra were
normalized using the probabilistic quotient normalization approach14 (link) with a median of all spectra as a reference
spectrum. We applied Pareto scaling on the data set for multivariate
analysis only.
Metabolite assignment was completed exploiting
reference data from the literature,9 (link),25 (link) the HMDB,15 (link) MMCD,16 (link) bbiorefcode-2-0-0
(Bruker, GmbH, Rheinstetten, Germany), and Chenomx NMR Suite 7.0 (Chenomx
Inc., Edmonton, Canada) spectral databases.
