Mastomys coucha Virus-Free Housing

Virus-free Mastomys coucha were obtained from Janvier Labs (Le Genest-Saint-Isle, France). At the DKFZ Mastomys coucha were housed under specified pathogen-free (SPF) conditions in individually ventilated cages (Tecniplast GR900) at 22+/-2˚C and 55+/-10% relative humidity in a light/dark cycle of 14/10 h. Mastomys were fed with mouse breeding diet and allowed access to water ad libitum. According to the three R rules of animal experimentation, the animals used in this exploratory study were subgroups (n=6 animals per group; groups: PBS, MnPV L1-VLPs, HPV16 RG1-VLPs, CUT-PANHPVAX) of a larger exploratory study also including testing the protective efficacy of an alternative L1 isoform which is published elsewhere (48 (link)).

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Ahmels M., Mariz F.C., Braspenning-Wesch I., Stephan S., Huber B., Schmidt G., Cao R., Müller M., Kirnbauer R., Rösl F, & Hasche D. (2022). Next generation L2-based HPV vaccines cross-protect against cutaneous papillomavirus infection and tumor development. Frontiers in Immunology, 13, 1010790.

Publication 2022

GR900

Animals Diet Hpv16 Humidity Isoform Mastomys Mouse Specified pathogen free Virus

Corresponding Organization : German Cancer Research Center

Other organizations : Medical University of Vienna

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Variable analysis

independent variables

Treatment groups: PBS, MnPV L1-VLPs, HPV16 RG1-VLPs, CUT-PANHPVAX

dependent variables

Not explicitly mentioned

control variables

Housing conditions: Individually ventilated cages, 22+/-2°C, 55+/-10% relative humidity, 14/10 h light/dark cycle
Diet: Mouse breeding diet, water ad libitum

controls

Positive control: Not mentioned
Negative control: PBS group

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