Bacteria were quantitated as described before.64 (link)67 (link, link, link) Briefly, each cornea was homogenized in 1.0 mL sterile saline containing 0.25% BSA; 0.1 mL corneal homogenate was serially diluted 1:10 in the same solution and selected dilutions were plated in triplicate on Pseudomonas isolation agar (Becton Dickinson, Sparks, MD, USA). Plates were incubated O/N at 37°C and the number of viable bacteria counted. Results are reported as log10 number of CFU/cornea ± SEM.
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Quantifying Bacterial Burden in Corneas
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Corresponding Organization : Kresge Eye Institute
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Variable analysis
independent variables
- Bacterial quantitation method
- Log10 number of CFU/cornea ± SEM
- Cornea homogenization in 1.0 mL sterile saline containing 0.25% BSA
- Serial dilution of 0.1 mL corneal homogenate in the same solution
- Plating of selected dilutions in triplicate on Pseudomonas isolation agar
- Incubation of plates O/N at 37°C
- Positive control: Not explicitly mentioned
- Negative control: Not explicitly mentioned
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