Retinal Imaging and Morphometry in Mice

After anesthesia (ketamine 80 mg/kg and xylazine 20 mg/kg), the right eye of the mouse was dilated with 0.125% atropine and treated with a drop of 2% Methocel® (OmniVision, Switzerland). Using an ophthalmoscope, the ocular fundus was focused and positioned circumferentially around the optic disc; FP and FFA images were then acquired using the Phoenix Micron III retinal imaging system (Tempe, AZ). An intraperitoneal injection of sodium fluorescein (10 mg/kg) is essential prior to FFA administration. Following the manufacturer’s instructions, SD-OCT images were scanned vertically or horizontally at the indicated positions. The thickness of the three retinal sublayers was automatically identified and computed using the InSight software. The inner sublayer comprises the nerve fiber layer, ganglion layer, inner plexiform layer (IPL), and inner nuclear layer (INL). The middle sublayer comprises the outer plexiform layer (OPL) and outer nuclear layer (ONL), whereas the outer sublayer comprises the inner and outer segments (IS/OS) of photoreceptors [55 (link)].