Isolation and Culture of Mouse Hepatocytes

Mouse hepatocyte primary cultures were established from the livers of adult Bsg^+/+ and Bsg^–/– mice according to the previously described method (44 (link)). In short, immediately following euthanasia of the animals, livers were perfused sequentially with buffered Liver Perfusion Medium (catalog 17701-038; Thermo Fisher Scientific) and Liver Digest Medium (catalog 17703-034; Thermo Fisher Scientific). Hepatocytes from the removed livers were extracted into William’s E Medium (catalog A1217601; Thermo Fisher Scientific) containing Primary Hepatocyte Thawing and Plating Supplements (catalog CM3000; Thermo Fisher Scientific). Primary cultured hepatocytes were maintained in William’s E Medium supplemented with Maintenance Supplements (catalog CM4000; Thermo Fisher Scientific).

Free full text: Click here

Ryuge A., Kosugi T., Maeda K., Banno R., Gou Y., Zaitsu K., Ito T., Sato Y., Hirayama A., Tsubota S., Honda T., Nakajima K., Ozaki T., Kondoh K., Takahashi K., Kato N., Ishimoto T., Soga T., Nakagawa T., Koike T., Arima H., Yuzawa Y., Minokoshi Y., Maruyama S, & Kadomatsu K. (2021). Basigin deficiency prevents anaplerosis and ameliorates insulin resistance and hepatosteatosis. JCI Insight, 6(20), e142464.

Publication 2021

Liver Digest Medium William’s E Medium

Adult Euthanasia of animals Hepatocytes Livers Mice Perfusion Supplements

Corresponding Organization :

Other organizations : Nagoya University, Keio University, Fujita Health University, National Institute for Physiological Sciences, Rakuwakai Otowa Hospital

Top 5 similar protocols

Variable analysis

independent variables

Genotype: Bsg+/+ (wild-type) and Bsg-/- (knockout) mice

dependent variables

Hepatocyte (liver cell) properties and behavior

control variables

Perfusion and extraction of hepatocytes from mouse livers using standardized methods and solutions
Maintenance of primary cultured hepatocytes in standardized culture medium

controls

Positive control: Hepatocytes from Bsg+/+ (wild-type) mice
Negative control: Hepatocytes from Bsg-/- (knockout) mice

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!