Cryopreserved PBMCs were used to evaluate the H3Ac in lymphocytes by flow cytometry as described previously13 (link). Briefly, after a 20 min blocking with PBS 10% FBS, cells were stained for 30 min with anti-acetyl histone H3 polyclonal rabbit (MerckMillipore 06–599) or with the control stain using normal rabbit serum (LifeTechnologies 10510). After washing, a 30 min incubation with the secondary antibody in the dark was performed (donkey anti-rabbit IgG, LifeTechnologies A21206). The median fluorescence intensity (MFI) for acetyl histone H3 stain was determined and the background was subtracted using the MFI of the isotype control staining.
Oriol-Tordera B., Esteve-Codina A., Berdasco M., Rosás-Umbert M., Gonçalves E., Duran-Castells C., Català-Moll F., Llano A., Cedeño S., Puertas M.C., Tolstrup M., Søgaard O.S., Clotet B., Martínez-Picado J., Hanke T., Combadiere B., Paredes R., Hartigan-O'Connor D., Esteller M., Meulbroek M., Calle M.L., Sanchez-Pla A., Moltó J., Mothe B., Brander C, & Ruiz-Riol M. (2022). Epigenetic landscape in the kick-and-kill therapeutic vaccine BCN02 clinical trial is associated with antiretroviral treatment interruption (ATI) outcome. EBioMedicine, 78, 103956.
Corresponding Organization : Centro de Investigación Biomédica en Red
Other organizations :
Universitat Autònoma de Barcelona, Centro Nacional de Análisis Genómico, Pompeu Fabra University, Institut d'Investigació Biomédica de Bellvitge, Josep Carreras Leukaemia Research Institute, Bellvitge University Hospital, Aarhus University Hospital, Inserm, Sorbonne Université, Centre d'Immunologie et des Maladies Infectieuses, Red de Investigación en Sida, Hospital Universitari Germans Trias i Pujol, Universitat de Vic - Universitat Central de Catalunya, Institució Catalana de Recerca i Estudis Avançats, Jenner Institute, University of Oxford, Kumamoto University, University of California, Davis, Veterinary Medical Teaching Hospital, Universitat de Barcelona, Centro de Investigación Biomédica en Red de Cáncer, Vall d'Hebron Institut de Recerca
Negative control: Normal rabbit serum (isotype control)
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