The following plasmids were obtained from Addgene: mApple-TOMM20-N-10 (Addgene; 54955), mCitrine-N1 (Addgene; 54594) (27 (link)), and EBFP2-Lysosomes-20 (Addgene; 55246) were gifts from M. Davidson; LAMP1-mGFP (Addgene; 34831) (28 (link)) was a gift from E. Dell-Angelica; pmTurquoise2-N1 (Addgene; 60561) (29 (link)) was a gift from D. Gadella; Halo-KDEL (Addgene; 124316) (30 (link)) was a gift from J. Wang; pEGFP-parkin WT (Addgene; 45875) (31 (link)) and pEGFP-parkin C431S (Addgene; 45877) (31 (link)) were gifts from E. Fon; EGFP-Rab7A (Addgene; 28047) (32 (link)) was a gift from Q. Zhong; pMXs-3xHA-EGFP-OMP25 (Addgene; 83356) (24 (link)) and pLJC5-Lamp1-RFP-3xHA (Addgene; 102932) (23 (link)) were gifts from D. Sabatini; psPAX2 (Addgene; 12260) was a gift from D. Trono; Lamp1-RFP (Addgene; 1817) (33 (link)) was a gift from W. Mothes; pGEX-4T-3-mR7BD (Addgene; 79149) (34 (link)) was a gift from A. Edinger; and Halo-TOMM20-N-10 (Addgene; 123284) was a gift from K. McGowan. Additional plasmids included the following: EGFP-Rab7 K38R (a gift from P. Song) (9 (link)), pLP3 (Invitrogen; K497500), and pGEX-4T-1 (Millipore Sigma; GE28-9545-49). The following plasmids were generated for this study using standard cloning procedures: LAMP1-Halo, LAMP1-pmTurquoise2, mCitrine-TOMM20, Halo-Parkin WT, Halo-Parkin C431S, pER4-mApple-TOMM20, pER4-LAMP1-mGFP, and pER4-3xHA-EGFP-OMP25.
Protocol detail
Plasmid Sourcing and Cloning for Organelle Imaging
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Corresponding Organization : Northwestern University
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Variable analysis
independent variables
- MApple-TOMM20-N-10
- MCitrine-N1
- EBFP2-Lysosomes-20
- LAMP1-mGFP
- PmTurquoise2-N1
- Halo-KDEL
- PEGFP-parkin WT
- PEGFP-parkin C431S
- EGFP-Rab7A
- PMXs-3xHA-EGFP-OMP25
- PLJC5-LAMP1-RFP-3xHA
- PGEX-4T-3-mR7BD
- Halo-TOMM20-N-10
- EGFP-Rab7 K38R
- LAMP1-Halo
- LAMP1-pmTurquoise2
- MCitrine-TOMM20
- Halo-Parkin WT
- Halo-Parkin C431S
- PER4-mApple-TOMM20
- PER4-LAMP1-mGFP
- PER4-3xHA-EGFP-OMP25
- Not explicitly mentioned
- PsPAX2
- Lamp1-RFP
- PGEX-4T-1
- Not specified
- Not specified
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