We assessed expression pattern of extracellular matrix proteins (type-II collagen, aggrecan, and cartilage oligomeric matrix protein, that is, COMP) and CD44 (a cell surface antigen) through immunostaining as described.4 (link) The primary antibodies used are shown in Table 1 . Images were captured on an Eclipse E800 microscope (Nikon, Tokyo, Japan) with QImaging Retiga 2000R Fast 1394 camera and MetaMorph software v7.7 (Molecular Devices, Sunnyvale, CA).
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Immunohistochemical Evaluation of ECM Proteins
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Aggrecan
Antibodies
Cartilage oligomeric matrix protein
Cd44
Cell surface antigen
Comp
Devices
Extracellular matrix proteins
Microscope
Type ii collagen
Corresponding Organization : Barnes-Jewish Hospital
Other organizations : The First Affiliated Hospital, Sun Yat-sen University, Sun Yat-sen University, Washington University in St. Louis
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Variable analysis
independent variables
- Type-II collagen
- Aggrecan
- Cartilage oligomeric matrix protein (COMP)
- CD44 (a cell surface antigen)
- Expression pattern of extracellular matrix proteins and CD44
- Not explicitly mentioned
- No positive or negative controls were explicitly mentioned in the input.
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