Lesion size was analyzed at 24 h and 14 d after surgery on MRI (Bruker Pharmascan Germany 7 Tesla horizontal bore magnets) using T2 maps (RARE 8 T2, 180° flip angle, 3 averages). Ten contiguous coronal slices (thickness: 1 mm) were acquired with a field of view of 35 X 35 mm and a matrix size of 256 X 256 [repetition time (TR) 3000 ms, echo time (TE) 29.5 ms, imaging time 25.5 min, 3 averages]. All images were processed using the J 1.42 Image program (NIH software). To correct for the brain edema effect, lesion size was determined by an indirect method: (infarct area) = (area of the intact contralateral hemisphere)–(area of the intact ipsilateral hemisphere) [2 (link),10 (link)].
Lesion size was also estimated with H&E staining of brain sections at 14 d. The samples were coronally sectioned in 10 μm thick slices and digitalized images were made of these slices (Epson Perfection 1260 scanner) and were used to automatically measure the ischemic area (Image Pro plus 4.0, Media Cybernetics, USA) [11 (link)]. To check whether H&E showed the infarct area, MAP-2 staining (polyclonal antibody diluted 1:1000, Millipore) and GFAP staining (monoclonal antibody diluted 1:400, Chemicon) was performed.
Lesion size was also estimated with H&E staining of brain sections at 14 d. The samples were coronally sectioned in 10 μm thick slices and digitalized images were made of these slices (Epson Perfection 1260 scanner) and were used to automatically measure the ischemic area (Image Pro plus 4.0, Media Cybernetics, USA) [11 (link)]. To check whether H&E showed the infarct area, MAP-2 staining (polyclonal antibody diluted 1:1000, Millipore) and GFAP staining (monoclonal antibody diluted 1:400, Chemicon) was performed.
Free full text: Click here
