LuCaP tissue microarrays (TMAs) were constructed and stained as previously described (16 (link)). Antibodies directed toward the detection of BCL2 [EPR17509] (Abcam, cat# ab182858) p-CDK1 (Abcam, ab-133463) and Wee1 (Santa Cruz Biotechnologies, sc-5258) were used according to the manufacturer’s instructions. Staining assessment was blinded and the H-score was calculated and averaged across multiple tissue/tumor cores for the same LuCaP PDX line.
Protocol detail
Antibody-Based LuCaP TMA Staining Protocol
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Corresponding Organization : University of Washington
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Variable analysis
independent variables
- Antibodies directed toward the detection of BCL2 [EPR17509] (Abcam, cat# ab182858)
- P-CDK1 (Abcam, ab-133463)
- Wee1 (Santa Cruz Biotechnologies, sc-5258)
- Staining assessment
- H-score calculated and averaged across multiple tissue/tumor cores for the same LuCaP PDX line
- LuCaP tissue microarrays (TMAs) were constructed and stained as previously described (16 (link))
- Positive controls
- Negative controls
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