Culturing Multiple Myeloma Cell Lines

Cell lines MM1.S (Dexamethasone sensitive), MM1.R (Dexamethasone resistant) and RPMI 8226 were kindly provided by Dr. Jonathan Keats (TGen, Phoenix, AZ, USA), DOX 40 (Doxorubicin resistant) was kindly provided by Dr. William Dalton (Moffitt Cancer Center, Tampa, FL, USA), and NC-H929 was purchased from ATCC (Manassas, VA, USA). Cells were cultured in RPMI 1640 media (Mediatech Inc., Manassas, VA, USA) containing 10% fetal bovine serum (Mediatech Inc.), 100U/mL penicillin, 100uG/ml streptomycin, and 2mM glutamine (Invitrogen, Grand Island, NY, USA). Freshly obtained bone marrow aspirates from MM patients were obtained after informed consent and processed to collect either myeloma cells or bone marrow stromal cells (BMSCs) as described earlier [49 (link)–51 (link)]. Patient cells were cultured as MM cell lines except that 20% fetal bovine serum was used instead of 10% fetal bovine serum.