Diverse Plasmodium Species Evaluation

Different Plasmodium species available in our laboratories were used in this study: P. falciparum (3D7, FCR3, W2, D6, Dd2, V1-S, Nigeria, Santa Lucia), P. vivax (South Vietnam IV), P. malariae (Uganda I), P. knowlesi (Nuri) and P. ovale (Nigeria I). DNA from 50 clinical samples available from the CDC molecular diagnostic parasitology reference laboratory (Dr. A. DaSilva) (10 non-malaria samples, 13 P. falciparum, 9 P. vivax, 1 P. malariae, 11 P. ovale, 2 P. falciparum/P. malariae, 1 P. vivax/P. ovale, 2 P. falciparum/P. ovale mixed infections and 1 P. knowlesi; parasitemia data not available) were used. In addition, a set of 69 P. falciparum positive samples from a study in Kenya were tested anonymously (microscopic parasitemia range of 1230–231,040 parasites/µl).

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Lucchi N.W., Narayanan J., Karell M.A., Xayavong M., Kariuki S., DaSilva A.J., Hill V, & Udhayakumar V. (2013). Molecular Diagnosis of Malaria by Photo-Induced Electron Transfer Fluorogenic Primers: PET-PCR. PLoS ONE, 8(2), e56677.

Publication 2013

Malaria Microscopic Mixed infections Molecular diagnostic Parasitemia Parasites Plasmodium

Corresponding Organization :

Other organizations : Centers for Disease Control and Prevention, Atlanta Research and Education Foundation, Centers for Disease Control and Prevention, Kenya Medical Research Institute, Division of Parasitic Diseases and Malaria, Center for Global Health

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Variable analysis

independent variables

Different Plasmodium species available in the laboratories: P. falciparum (3D7, FCR3, W2, D6, Dd2, V1-S, Nigeria, Santa Lucia), P. vivax (South Vietnam IV), P. malariae (Uganda I), P. knowlesi (Nuri) and P. ovale (Nigeria I)

dependent variables

Parasitemia data (not available for clinical samples)

control variables

10 non-malaria samples

controls

Positive controls: P. falciparum (13 samples), P. vivax (9 samples), P. malariae (1 sample), P. ovale (11 samples), P. falciparum/P. malariae (2 samples), P. vivax/P. ovale (1 sample), P. falciparum/P. ovale (2 samples), P. knowlesi (1 sample)
Negative control: 10 non-malaria samples

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