Xenograft Mouse Model for Cancer Research

Female NCG (NOD/ShiLtJGpt-Prkdc^em26Cd52Il2rg^em26Cd22/Gpt) mice were purchased from Jiangsu Gempharmatech. NCG mice were maintained in accordance with the guidelines for laboratory animals approved by the Animal Research Committee of the University of Science and Technology of China and housed in an ultraclean barrier facility. The K562 (RRID:CVCL_0004) cell line was purchased from the Cell Bank of the Chinese Academy of Sciences (Shanghai, China). The Ho8910-Luc cell line was constructed and cryopreserved in our laboratory [29 (link)]. All cell lines were maintained in modified RPMI medium (HyClone, SH30809.01) supplemented with 10% FBS (Biological Industries, Beit HaEmek, Israel), 100 U/mL penicillin and 100 µg/mL streptomycin (Sangon Biotech, Shanghai, China) at 37 °C in a 5% CO₂ incubator. All the cell lines were identified with STR profiling by Genewiz cpmpany (Suzhou, China).