Western Blotting Analysis of Signaling Proteins

Western blotting was conducted according to a protocol described elsewhere (19 (link)). Proteins of interest were investigated with the primary antibodies to p-AMPK (ab133448), AMPK (ab207442) from Abcam (Cambridge, UK), p-Akt (Thr-308, 13038), Akt (9272), p-IRS-1 (Ser1101, 2385S), p-IRS-1 (Ser302, 2384S), IRS-1 (2382), p-CREB (9198S) and CREB (9197S) from the Cell Signaling Technology (Boston, USA). The signal was detected with chemiluminescent detection system (E412-01-AA, Western Lightning ECL; Vazyme). The signal was collected with a CCD camera system (Light-Capture; ATTO, Tokyo, Japan) and the statistical analysis was conducted with the ImageJ program.

Free full text: Click here

Cao X., Ye X., Zhang S., Wang L., Xu Y., Peng S., Zhou Y., Peng Y., Li J., Zhang X., Han X., Huang W.Y., Jia W, & Ye J. (2021). ADP Induces Blood Glucose Through Direct and Indirect Mechanisms in Promotion of Hepatic Gluconeogenesis by Elevation of NADH. Frontiers in Endocrinology, 12, 663530.

Publication 2021

CREB (9197S)

Antibodies Irs 1 Light Proteins

Corresponding Organization : Shanghai Jiao Tong University

Other organizations : Shanghai Ocean University, Nanjing Medical University, Jiangsu Province Hospital, Jiangxi Normal University

Top 5 similar protocols

Variable analysis

independent variables

Primary antibodies used: p-AMPK (ab133448), AMPK (ab207442), p-Akt (Thr-308, 13038), Akt (9272), p-IRS-1 (Ser1101, 2385S), p-IRS-1 (Ser302, 2384S), IRS-1 (2382), p-CREB (9198S) and CREB (9197S)

dependent variables

Protein levels of p-AMPK, AMPK, p-Akt, Akt, p-IRS-1, IRS-1, p-CREB and CREB

control variables

Chemiluminescent detection system (E412-01-AA, Western Lightning ECL; Vazyme)
CCD camera system (Light-Capture; ATTO, Tokyo, Japan)
ImageJ program for statistical analysis

controls

No positive or negative controls were explicitly mentioned in the input.

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!