Sperm Motility Analysis in Hgsnat Mice

Cauda epididymides of 5 Hgsnat+/+ and 8 Hgsnat−/− mice at 11 months were submerged in Medium 199, Hanks’ Balanced Salts (Gibco, cat# 12350–039). Epididymides were cut and squeezed gently to allow spermatozoa to diffuse into suspension for 10 min at 37°C. Sperm concentration was determined with a hemocytometer and then adjusted to 5 × 10⁶ cells/ml in Medium 199, Hanks’ Balanced Salts. Sperm motility parameters were determined using a computer-assisted sperm analysis system (CASA) with Sperm Vision HR software version 1.01 (Minitube, Ingersoll, ON, Canada) [83 (link)]. Two hundred spermatozoa were examined for each sample to determine each sperm motility parameter. Mann-Whitney tests (alternative nonparametric test) were done using Version 8 of Statistica for Windows (Statsoft, Inc., Tulsa, OK). The p values less than 0.05 were considered significant.