The concentration of H2O2 was evaluated based on a modified method (Velikova et al., 2000 (link)) originally described by Kazerooni et al. (2021 (link)). The powdered leaf tissue (0.3 g) was homogenized in an ice bath with 0.1% trichloroacetic acid (TCA; 5 ml). The absorbance of the mixture was recorded at 390 nm using a spectrophotometer.
López-Serrano et al. (2019 (link)) method was applied to evaluate the lipid content in soybean and maize leaves. The mixture comprising 0.1% TCA (0.5 ml) extract was added to 0.5% TBA (1 ml; prepared in 20% TCA). The reaction was commenced by incubating the mixture at 95°C for 30 min and terminated by placing the mixture in an ice bath for 10 min. Then the mixture was immediately centrifuged at 12,000 rpm for 5 min. Lipid content was measured at wavelengths of 532 and 600 nm (Thermo Fisher Scientific, Waltham, MA, USA).
We used a previously published method (Poirier et al., 1999 (link)) to determine the fatty acid content in soybean and maize leaves. Gas chromatography–mass spectrometry analysis was conducted on an Agilent Model 7890A series (Agilent, Dover, DE, USA).
López-Serrano et al. (2019 (link)) method was applied to evaluate the lipid content in soybean and maize leaves. The mixture comprising 0.1% TCA (0.5 ml) extract was added to 0.5% TBA (1 ml; prepared in 20% TCA). The reaction was commenced by incubating the mixture at 95°C for 30 min and terminated by placing the mixture in an ice bath for 10 min. Then the mixture was immediately centrifuged at 12,000 rpm for 5 min. Lipid content was measured at wavelengths of 532 and 600 nm (Thermo Fisher Scientific, Waltham, MA, USA).
We used a previously published method (Poirier et al., 1999 (link)) to determine the fatty acid content in soybean and maize leaves. Gas chromatography–mass spectrometry analysis was conducted on an Agilent Model 7890A series (Agilent, Dover, DE, USA).
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