Efficient Blood Extraction and Analysis

Example 8

The SFME sample processing can be done in fused silica tubing of smaller diameter which are commonly used as liquid line in liquid chromatography system (e.g., tubing having an inner diameter of 500 μm or less). The extraction can be induced by applying a push and pull force on one side of the tubing. The extract can be either directly analyzed by nanoESI or stored for further operations.

FIG. 10 show analysis of 50 ng/mL amitriptyline in bovine whole blood. MS/MS spectrum of the molecular ion was collected. The blood sample was first 10× diluted using H₂O as a reduction of viscosity. For extraction, 5 μL of the diluted sample was processed in a fused silica tubing (i.d. 500 μm) using methods of the invention. The extract was then infused into a nanoESI emitter and analyzed by nanoESI.

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US11875983B2. Systems and methods for quantifying an analyte extracted from a sample (2024-01-16). Purdue Research Foundation [US]. Inventors: Zheng Ouyang [US], Yue Ren [IN], Ziqing Lin [US].

Patent 2024

Amitriptyline Blood Bovine Liquid chromatography Ms ms Silica Viscosity

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Variable analysis

independent variables

Applying a push and pull force on one side of the tubing

dependent variables

Analysis of 50 ng/mL amitriptyline in bovine whole blood
MS/MS spectrum of the molecular ion

control variables

Fused silica tubing of smaller diameter (e.g., inner diameter of 500 μm or less)
10× dilution of the blood sample using H2O to reduce viscosity

controls

Positive control: Not mentioned
Negative control: Not mentioned

Annotations

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