ChIP-seq Data Processing and Analysis

ChIP-seq libraries were prepared using the TruSeq ChIP Library Prep kit (Illumina) and subjected to paired-end sequencing. Quality control and pre-processing of raw data were processed using the Fastp software (version 0.20.1). Reads were mapped to the hg19 genome (ftp://ftp.ccb.jhu.edu/pub/data/bowtie2_indexes/hg19.zip) with Bowtie 2 44 (link) and duplicates were removed using the Picard v.2.20 tool 45 (link). MACS14 (version 1.4.2) was used for peak calling (q value ≤ 1e-04) 46 . The Chipseeker 47 (link) package in R statistical environment (R x64 3.6.3) was used to annotate the genomic region of the peaks. The motif enrichment analysis was performed using homer HOMER v4.11.1 (http://homer.ucsd.edu/homer/motif/).