Comprehensive Fungal Characterization Protocol
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Corresponding Organization : Westerdijk Fungal Biodiversity Institute
Other organizations : University of the Free State, Wageningen University & Research
Protocol cited in 4 other protocols
Variable analysis
- Protocols described by Leslie & Summerell (2006)
- Potato dextrose agar (PDA; recipe in Crous et al. 2009)
- Synthetic nutrient-poor agar (SNA; Nirenberg 1976)
- Carnation leaf agar (CLA; Fisher et al. 1982)
- Incubation temperature of 24 °C
- Light/dark cycle of 12/12 h cool fluorescent light/dark
- Colony morphology
- Pigmentation
- Odour
- Growth rates
- Micromorphological characters
- Mounting medium (water) for micromorphological examination
- Microscopes (Zeiss Axioskop 2 plus with DIC optics, Nikon AZ100 stereomicroscope)
- Nikon DS-Ri2 high definition color digital cameras for photodocumentation
- Nikon software NIS-elements D v. 4.50 for measurements
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