Cy5.5-labeled Peptide Synthesis

The peptides were synthesized using a PS3 automatic synthesizer (Protein Technologies Inc, Tucson, AZ) and labeled with Cy5.5, a near-infrared red (NIR) fluorophore (cyanine5.5 NHS ester, #27020; Lumiprobe, Hunt Valley, MD ), on the C-terminus using a GGGSK linker (14 (link)). The peptides were purified to >99% by HPLC and lyophilized for storage at −80 °C.

Partial Protocol Preview
This section provides a glimpse into the protocol.
The remaining content is hidden due to licensing restrictions, but the full text is available at the following link: Access Free Full Text.

Wang F., Duan X., Chen J., Gao Z., Zhou J., Wu X., Chang T.S., Lee M., Li G., Nusrat A., Kuick R., Appelman H.D, & Wang T.D. (2020). Integrated Imaging Methodology Detects Claudin-1 Expression in Premalignant Nonpolypoid and Polypoid Colonic Epithelium in Mice. Clinical and Translational Gastroenterology, 11(1), e00089.

Publication 2020

PS3 automatic synthesizer cyanine5.5 NHS ester

Cy5 5 Ester Hplc Peptides Protein

Corresponding Organization : University of Michigan–Ann Arbor

Top 5 similar protocols

Variable analysis

independent variables

Synthesis method: PS3 automatic synthesizer (Protein Technologies Inc, Tucson, AZ)

dependent variables

Degree of labeling with Cy5.5 (a near-infrared red (NIR) fluorophore)

control variables

Purity of the peptides (>99% by HPLC)
Storage conditions (-80 °C)

controls

No positive or negative controls were explicitly mentioned.

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!