Cytotoxicity of Nano-CuO and Nano-TiO2

The cytotoxicity of Nano-CuO and Nano-TiO₂ in BEAS-2B cells were determined by both CellTiter 96® AQueous Non-Radioactive Cell Proliferation Assay (MTS assay) (Promega, Madison, WI) and alamarBlue™ Cell Viability Reagent (alamarBlue assay) (Invitrogen, Eugene, OR) as described in our previous studies (Mo et al. 2009 (link); Yu et al. 2010 (link)). Briefly, 3x10³ BEAS-2B cells per well were seeded in 96-well plates. After 12 h incubation, cells were treated with different doses of Nano-CuO or Nano-TiO₂ (0, 0.5, 1.0, 1.5, 2.0, and 5.0 μg/mL) in a total volume of 200 μL per well. Cells without treatment were used as control. After 24 h treatment, the cytotoxicity was determined by recording the colorimetric absorbance at 490 nm for MTS assay and fluorescence at ex530/em590 for alamarBlue assay. The cell viability was presented as the percentage as compared to the control.

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Zhang Y., Mo Y., Yuan J., Zhang Y., Mo L, & Zhang Q. (2022). MMP-3 activation is involved in copper oxide nanoparticle-induced epithelial-mesenchymal transition in human lung epithelial cells. Nanotoxicology, 15(10), 1380-1402.

Publication 2021

CellTiter 96® AQueous Non-Radioactive Cell Proliferation Assay (MTS assay) alamarBlue™ Cell Viability Reagent

Alamarblue Assay Cell proliferation Cell viability Cells Colorimetric Cytotoxicity Fluorescence Nano tio2 Promega Radioactive

Corresponding Organization : University of Louisville

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Variable analysis

independent variables

Nano-CuO concentration
Nano-TiO2 concentration

dependent variables

Cell viability as a percentage of control

control variables

BEAS-2B cell line
Seeding density of 3x10^3 cells per well
Incubation time of 12 hours
Treatment duration of 24 hours
Total volume of 200 μL per well

controls

Cells without treatment (negative control)

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