Characterization of Human Amniotic Mesenchymal Stem Cells

The hAMSCs were extracted as previous described [15 (link)]. Third-passage (P3) cells were harvested from phenotype identification through staining with antibodies against CD34 (581, BD Pharmingen, USA), CD45 (HI30, BD Pharmingen, USA), HLA-DR (G46-6, BD Pharmingen, USA), CD11b (ICRF44, BD Pharmingen, USA), CD90 (5E10, BD Pharmingen, USA), CD73 (AD2, BD Pharmingen, USA) and CD105 (SN6, ebioscience, USA), and then analyzed by flow cytometer (BD FACS Canto II). The cells were checked for their multilineage differentiation using a specific induction medium (BGscience, China), and stained with Oil Red-O and Alizarin Red for adipogenesis and osteogenic differentiation, respectively. hAMSCs at passages 3 to 6 were used for the experiments.