Western blot analyses of protein samples were performed as described previously using rabbit anti-VSV-G (diluted 1:5,000; Sigma), rabbit anti-Hcp (diluted 1:5,000) and detected with anti-rabbit horseradish peroxidase-conjugated secondary antibodies (diluted 1:5,000; Sigma)6 (link). Western blots were developed using chemiluminescent substrate (Clarity Max, Bio-Rad or SuperSignal™ West Femto Maximum Sensitivity Substrate, Thermofisher) and imaged with the ChemiDoc Imaging System (Bio-Rad).
Protocol detail
Protein Detection via Western Blot
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Corresponding Organization :
Other organizations : McMaster University, Massachusetts Institute of Technology, University of Toronto
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Variable analysis
independent variables
- Rabbit anti-VSV-G antibody dilution (1:5,000)
- Rabbit anti-Hcp antibody dilution (1:5,000)
- Protein expression levels detected by Western blot
- Anti-rabbit horseradish peroxidase-conjugated secondary antibody dilution (1:5,000)
- Chemiluminescent substrate (Clarity Max, Bio-Rad or SuperSignal™ West Femto Maximum Sensitivity Substrate, Thermofisher)
- Imaging with ChemiDoc Imaging System (Bio-Rad)
- Positive control: Not specified
- Negative control: Not specified
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