HPAEC (cat #PCS-100-022) and HUVEC (cat#PCS-100-013) cells were purchased from ATCC and cultured in Vascular Cell Basal Medium (cat#PCS-100-030) supplemented with VEGF (Endothelial Cell Growth Kit-VEGF, cat#PCS-100-041). Normal blood donor (NBD) peripheral blood samples were collected in Streck cfDNA blood collection tubes at the Scripps NBD Service and processed as previously published [28 (link),29 (link)]. Cell-line cells were spiked into the NBD sample at 100 cell/mL (HUVEC) and 430 cells/mL (HPAECs).
Welter L., Zheng S., Setayesh S.M., Morikado M., Agrawal A., Nevarez R., Naghdloo A., Pore M., Higa N., Kolatkar A., Thiele J.A., Sharma P., Moore H.C., Richer J.K., Elias A., Pienta K.J., Zurita A.J., Gross M.E., Shishido S.N., Hicks J., Velasco C.R, & Kuhn P. (2023). Cell State and Cell Type: Deconvoluting Circulating Tumor Cell Populations in Liquid Biopsies by Multi-Omics. Cancers, 15(15), 3949.
Other organizations :
University of Kansas Medical Center, Cleveland Clinic, University of Colorado Denver, University of Colorado Anschutz Medical Campus, University of Colorado Cancer Center, Johns Hopkins University, Johns Hopkins Medicine, Larry Ellison Foundation
Culture of cells in Vascular Cell Basal Medium (cat#PCS-100-030)
Normal blood donor (NBD) peripheral blood samples collected in Streck cfDNA blood collection tubes
Spiking of cell-line cells into the NBD sample at 100 cells/mL (HUVEC) and 430 cells/mL (HPAECs)
controls
Positive control: Not explicitly mentioned
Negative control: Not explicitly mentioned
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