Culturing Gastric Cancer Cell Lines

Gastric cancer cell lines, BGC-823, SGC7901, AGS, MKN28, and HGC-27, and normal human gastric epithelial cells, GES-1, were obtained from Procell Life Science&Technology Co,.Ltd. (Wuhan, China) (http://www.procell.com.cn/). The cells other than AGS were cultured in Roswell Park Memorial Institute (RPMI)-1640 medium (Gibco, Carlsbad, CA, USA) supplemented with 10% fetal bovine serum (FBS; Procell Life Science & Technology Co,. Ltd.) and 100 U/mL penicillin streptomycin solution. AGS cells were cultured in F12 medium containing 10% FBS and 100 U/mL penicillin streptomycin solution. All cells were maintained at 37 °C in a saturated humidity atmosphere containing 95% air and 5% CO₂. Cells were passaged when cell density reached 90%. After that, the medium was discarded and 1 mL sterile PBS was used to wash the Petri dish, and the cells were trypsinized and resuspended prior to cell passage [26 (link)].