Nalm6 and HEK293 Cell Culture Protocol

The Nalm6 32 (link) has been previously described. RPMI 1640 medium (Cellgro, USA) supplemented with 10% fetal bovine serum (Hyclone, USA) and DMEM (Cellgro) supplemented with 10% fetal calf serum and 1% L-glutamine (Cellgro) were used for culture of Nalm6 and HEK293 cell, respectively, incubated at 37°C in a humidified atmosphere of 5% CO2. Primary human B-ALL were cultured in RPMI 1640 medium (Cellgro) supplemented with 10% fetal bovine serum (Hyclone). CX4945 was obtained from Sigma (St. Louis, MO, USA). Cells were cultured with or without CX4945 and collected for total RNA isolation. Human IKZF1 retroviral construct and retroviral production were described previously 14 (link), 15 (link), 19 (link), 20 (link).

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Han Q., Ma J., Gu Y., Song H., Kapadia M., Kawasawa Y.I., Dovat S., Song C, & Ge Z. (2019). RAG1 high expression associated with IKZF1 dysfunction in adult B-cell acute lymphoblastic leukemia. Journal of Cancer, 10(16), 3842-3850.

Publication 2019

RPMI 1640 medium DMEM fetal calf serum L-glutamine CX4945

Atmosphere Cells Cultured medium Cx4945 Fetal bovine serum Glutamine Hek293 cell Human Isolation Retroviral

Corresponding Organization :

Other organizations : Zhongda Hospital Southeast University, Southeast University, Penn State Milton S. Hershey Medical Center, Pennsylvania State University, Eastern Cooperative Oncology Group

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Variable analysis

independent variables

CX4945

dependent variables

Total RNA isolation

control variables

RPMI 1640 medium (Cellgro, USA) supplemented with 10% fetal bovine serum (Hyclone, USA)
DMEM (Cellgro) supplemented with 10% fetal calf serum and 1% L-glutamine (Cellgro)
Incubation at 37°C in a humidified atmosphere of 5% CO2

positive controls

Nalm6 cell line
HEK293 cell line

negative controls

Primary human B-ALL cells cultured in RPMI 1640 medium (Cellgro) supplemented with 10% fetal bovine serum (Hyclone)

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