Sample preparation for proteomic analysis was performed as described previously [13 (link)]. Peptides were resuspended in 5% formic acid and analyzed by liquid chromatography tandem mass spectrometry (MS/MS). Ten microliters was injected onto a C18 trap column (Thermo Scientific Acclaim), flushed into an analytical column (Agilent), and eluted via a mobile phase gradient of 5%–80% solvent B over 120 minutes. The eluted material was directly applied to the nanospray source of a QSTAR Elite instrument (Applied Biosystems), and data analysis was conducted with Analyst 2.0 (Applied Biosystems).
Protocol detail
Proteomic Analysis by LC-MS/MS
Partial Protocol Preview
This section provides a glimpse into the protocol.
The remaining content is hidden due to licensing restrictions, but the full text is available at the following link:
Access Free Full Text.
Corresponding Organization : Australian Institute of Tropical Health and Medicine
Other organizations : Graduate School USA, University of Cambridge, University of Queensland, QIMR Berghofer Medical Research Institute, University of Technology Sydney, George Washington University, Khon Kaen University
Top 5 similar protocols
Variable analysis
independent variables
- Sample preparation protocol
- Peptide identification and quantification by liquid chromatography tandem mass spectrometry (MS/MS)
- Injection volume (10 microliters)
- C18 trap column (Thermo Scientific Acclaim)
- Analytical column (Agilent)
- Mobile phase gradient (5%–80% solvent B over 120 minutes)
- QSTAR Elite instrument (Applied Biosystems)
- Analyst 2.0 software (Applied Biosystems)
Annotations
Based on most similar protocols
Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.
Sign up for free or login to display all annotations
As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!