Transfection of Plasmids Expressing Proteins and shRNAs

Plasmids expressing LMAN2, SIRT, HDAC, or GFP proteins^{13 (link),21 (link)} were transfected using Lipofectamine 2000, according to the manufacturer’s instruction.
pLKO-based Validated MISSION shRNAs (TRCN0000040221, TRCN0000040222) targeting human SIRT2 gene for human cells and negative control (SHC001) were purchased from Sigma. Plasmids carrying U6 promoter-driven shRNAs were transfected using Lipofectamine 2000, according to the manufacturer’s instruction.

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Lee B.R., Sanstrum B.J., Liu Y, & Kwon S.H. (2019). Distinct role of Sirtuin 1 (SIRT1) and Sirtuin 2 (SIRT2) in inhibiting cargo-loading and release of extracellular vesicles. Scientific Reports, 9, 20049.

Publication 2019

Lipofectamine 2000 SHC001

Cells Human Lipofectamine 2000 Plasmids Shrnas Sirt Sirt2

Corresponding Organization :

Other organizations : Augusta University

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Variable analysis

independent variables

Plasmids expressing LMAN2, SIRT, HDAC, or GFP proteins
PLKO-based Validated MISSION shRNAs (TRCN0000040221, TRCN0000040222) targeting human SIRT2 gene for human cells
Negative control (SHC001) shRNA

dependent variables

Not explicitly mentioned

control variables

Lipofectamine 2000 used for transfection, according to the manufacturer's instruction

controls

Positive control: Not specified
Negative control: Negative control (SHC001) shRNA

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