Xenograft model of neuroblastoma

Female BALB/c nude mice (4 weeks old) received a subcutaneous injection of NB cells (2 × 10⁶ cells per mouse) suspended in 100 μl of extracellular matrix gel (Corning, NY, United States) into their front flanks. The mice were randomly divided into two groups (n = 5) and treated with CYN once a day for 3 weeks (0 or 5 mg/kg, i.g.). This concentration was referred to the article by Zhang et al. (Zheng et al., 2020 (link)). Tumour volumes and mouse weights were measured every 3 days. Tumour volumes were calculated with the formula (length × width × height)/2. After 3 weeks, all the mice were sacrificed by euthanasia, and afterwards, the tumours were quickly collected for weight measurements and then embedded in paraffin for immunohistochemistry.