Cells were seeded in wells of 6-well plates with 15×104cells/well and incubated in DMEM containing 10% FBS. The OAS1-siRNAs were used to impair OAS1 expression for 48 h, then cells were treated with 10 μM cisplatin for 24h. Finally, cells were digested and re-suspension with 1×bingding buffer, then, cells were treated with Annexin V-FITC (10 μl) and PI (5μl) (Absin, China) (26 (link)).
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