APC was synthesized as described previously (19 (link)). In brief, 1 mg of anti-mouse/human CD44 monoclonal antibody (6.7 nmol; clone IM7; Bio X Cell, West Lebanon, NH, USA) was incubated with five-fold molar excess of IR700 NHS ester (10 mM in DMSO; LI-COR Biosciences, Lincoln, NE, USA) in 100 mM Na2HPO4 solution (pH 8.5) for one hour at room temperature. The mixture was purified with PD-10 columns containing Sephadex G25 resin (GE Healthcare, Piscataway, NJ, USA). The resulting APC was abbreviated as CD44-IR700.
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Synthesis and Purification of CD44-IR700
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Other organizations : Center for Cancer Research, National Cancer Institute
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- Incubation of 1 mg of anti-mouse/human CD44 monoclonal antibody (6.7 nmol; clone IM7; Bio X Cell, West Lebanon, NH, USA) with five-fold molar excess of IR700 NHS ester (10 mM in DMSO; LI-COR Biosciences, Lincoln, NE, USA)
- Resulting APC, abbreviated as CD44-IR700
- 100 mM Na2HPO4 solution (pH 8.5)
- Incubation time of one hour at room temperature
- Purification of the mixture with PD-10 columns containing Sephadex G25 resin (GE Healthcare, Piscataway, NJ, USA)
- No positive or negative controls were explicitly mentioned.
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