BALB/c and MIF-deficient mice on the BALB/c background were bred in-house and housed in individually-ventilated cages (IVCs) according to UK Home Office guidelines. The Mif−/− construct contains a neomycin cassette disrupting exons 2 and 3 of the Mif gene75 (link). ACKR3-GFP mice (Ackr3tm1Litt)82 (link) were kindly provided by Prof. Gerard Graham, University of Glasgow, UK.
Infections employed N. brasiliensis maintained as previously described83 . NES was collected as spent culture medium collected over 7 days from adult N brasiliensis as described84 (link), centrifuged at 400 g for 10 min to remove eggs, and diafiltrated into PBS over a 3000 MW cut-off membrane in an Amicon, sterilized by passage through a 0.2 μm filter (Millipore) and frozen at −80 °C.
Adult worm counts were conducted after small intestines were removed and sliced longitudinally. Egg counts were performed on 3–4 fecal pellets which were weighed and resuspended in 2 ml dH2O; 2 ml of saturated salt solution (400 g NaCl in 1 L dH2O) was then added and eggs enumerated using a McMaster egg counting chamber. Egg counts are represented as eggs/g fecal material.
Infections employed N. brasiliensis maintained as previously described83 . NES was collected as spent culture medium collected over 7 days from adult N brasiliensis as described84 (link), centrifuged at 400 g for 10 min to remove eggs, and diafiltrated into PBS over a 3000 MW cut-off membrane in an Amicon, sterilized by passage through a 0.2 μm filter (Millipore) and frozen at −80 °C.
Adult worm counts were conducted after small intestines were removed and sliced longitudinally. Egg counts were performed on 3–4 fecal pellets which were weighed and resuspended in 2 ml dH2O; 2 ml of saturated salt solution (400 g NaCl in 1 L dH2O) was then added and eggs enumerated using a McMaster egg counting chamber. Egg counts are represented as eggs/g fecal material.
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