Sodium lactate (lactate), 2,2′-azobis(2-methylpropionitrile)
(AIBN), methacrylic acid (MAA), ethylene glycol dimethacrylate (EGDMA),
phosphate-buffered saline (PBS) tablets (pH 8.0 ± 0.02, 0.1 M),
anhydrous dichloromethane >99.8% purity containing 40–150
ppm
amylene as a stabilizer, and anhydrous methanol of 99.8% purity were
all obtained from Sigma-Aldrich and used in the synthesis of both
MIP and NIP powders. All chemicals were used as received with the
exception of MAA and EGDMA, which had inhibitors removed by gravity
filtration via prepacked aluminum oxide (activated, basic) columns
designed for the removal of hydroquinone and monomethyl ether hydroquinone.
Deionized (DI) water and ultrapure water (resistivity of 18 MΩ
cm–1) were produced using a Purelab Chorus 1 Complete
water purification system (Elga LabWater, Veolia Water Systems Ltd.).
Polymer powders were synthesized by first decanting aluminum oxide
into two syringes filled with glass fibers. MAA as a functional monomer
and EGDMA as a cross-linker were filtered (separately) through the
columns under gravity. Once the inhibitors were removed, MAA (0.24
mL, 2.4 mmol) and EGDMA (2.26 mL, 12.0 mmol) were combined in a reaction
vessel with the addition of 4 mL of dichloromethane. Lactate (44.8
mg, 0.4 mmol) as the template molecule was then added and solubilized
with the addition of 0.4 mL of anhydrous methanol. After the reaction
vessel was concealed from light, AIBN (16.4 mg, 0.1 mmol) was added
as the initiator. The mixture was degassed with nitrogen for 25 min
and then photochemically polymerized for 30 h using a standard laboratory
UV reactor (Cole-Parmer 4-W UV lamp) at a wavelength of 365 nm and
kept at 4 °C using a recirculatory bath (Isotemp). The obtained
colorless and translucent polymer was manually ground using a pestle
and mortar, mechanically milled (Retsch MM 400) at a frequency of
25 Hz for 25 min, and sieved using a 0.25 mm mesh.
(AIBN), methacrylic acid (MAA), ethylene glycol dimethacrylate (EGDMA),
phosphate-buffered saline (PBS) tablets (pH 8.0 ± 0.02, 0.1 M),
anhydrous dichloromethane >99.8% purity containing 40–150
ppm
amylene as a stabilizer, and anhydrous methanol of 99.8% purity were
all obtained from Sigma-Aldrich and used in the synthesis of both
MIP and NIP powders. All chemicals were used as received with the
exception of MAA and EGDMA, which had inhibitors removed by gravity
filtration via prepacked aluminum oxide (activated, basic) columns
designed for the removal of hydroquinone and monomethyl ether hydroquinone.
Deionized (DI) water and ultrapure water (resistivity of 18 MΩ
cm–1) were produced using a Purelab Chorus 1 Complete
water purification system (Elga LabWater, Veolia Water Systems Ltd.).
Polymer powders were synthesized by first decanting aluminum oxide
into two syringes filled with glass fibers. MAA as a functional monomer
and EGDMA as a cross-linker were filtered (separately) through the
columns under gravity. Once the inhibitors were removed, MAA (0.24
mL, 2.4 mmol) and EGDMA (2.26 mL, 12.0 mmol) were combined in a reaction
vessel with the addition of 4 mL of dichloromethane. Lactate (44.8
mg, 0.4 mmol) as the template molecule was then added and solubilized
with the addition of 0.4 mL of anhydrous methanol. After the reaction
vessel was concealed from light, AIBN (16.4 mg, 0.1 mmol) was added
as the initiator. The mixture was degassed with nitrogen for 25 min
and then photochemically polymerized for 30 h using a standard laboratory
UV reactor (Cole-Parmer 4-W UV lamp) at a wavelength of 365 nm and
kept at 4 °C using a recirculatory bath (Isotemp). The obtained
colorless and translucent polymer was manually ground using a pestle
and mortar, mechanically milled (Retsch MM 400) at a frequency of
25 Hz for 25 min, and sieved using a 0.25 mm mesh.
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