Evaluating Cancer Stem-like Cells via Spheroid Formation

Spheroid formation assays were performed to evaluate the regulation of cancer stem-like cells (CSCs). OSCC cells were dissociated and suspended in spheroid medium consisting of serum-free DMEM/F12 (Gibco, USA), human bFGF (20 ng/ml, Sino Biological Inc., China), human EGF (20 ng/ml, Sino Biological Inc., China), and B-27 supplement (Life Technologies, USA) in 6-well ultra-low attachment dishes (Corning, USA) under different treatment for 10 days [15 (link)]. The spheroid morphology was observed microscopically, and the number of spheroids was counted and compared.

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You Y., Tian Z., Du Z., Wu K., Xu G., Dai M., Wang Y, & Xiao M. (2022). M1-like tumor-associated macrophages cascade a mesenchymal/stem-like phenotype of oral squamous cell carcinoma via the IL6/Stat3/THBS1 feedback loop. Journal of Experimental & Clinical Cancer Research : CR, 41, 10.

Publication 2022

DMEM/F12 human bFGF human EGF B-27 supplement 6-well ultra-low attachment dishes

Assays Biological Cancer stem cells Cells Dishes Human Serum Sino Supplement

Corresponding Organization : Shanghai Stomatological Hospital

Other organizations : Shanghai Ninth People's Hospital, Shanghai Jiao Tong University

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Variable analysis

independent variables

Different treatment conditions

dependent variables

Spheroid morphology
Number of spheroids

control variables

OSCC cells
Spheroid medium (serum-free DMEM/F12, human bFGF (20 ng/ml), human EGF (20 ng/ml), and B-27 supplement)
6-well ultra-low attachment dishes
10 days of culture

controls

No positive or negative controls were explicitly mentioned in the input.

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