Phages were transferred onto electron microscopy (EM) grids (pioloform-coated 100 mesh, Agar Scientific) by floating the grids on droplets of virus suspension for 3 min, washed four times for 3 min on droplets of deionized water, before negative staining on droplets of 2 % w/v uranyl acetate for 3 min. Excess stain was removed using filter paper. After air-drying, samples were visualized using a JEOL JEM 1400 transmission electron microscope operated at 120 kV and images were taken with a digital camera (Gatan ES 1000W, Ametek).
Protocol detail
Negative Staining of Phage Samples
Partial Protocol Preview
This section provides a glimpse into the protocol.
The remaining content is hidden due to licensing restrictions, but the full text is available at the following link:
Access Free Full Text.
Corresponding Organization :
Other organizations : University of Exeter, University of the West of England, Royal Devon and Exeter Hospital
Top 5 similar protocols
Variable analysis
independent variables
- Duration of floating the grids on virus suspension droplets (3 min)
- Duration of washing the grids on deionized water droplets (4 times for 3 min each)
- Duration of negative staining on uranyl acetate droplets (3 min)
- Visualization of phages using transmission electron microscope
- Images captured using digital camera
- Pioloform-coated 100 mesh EM grids
- Concentration of uranyl acetate stain (2% w/v)
Annotations
Based on most similar protocols
Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.
Sign up for free or login to display all annotations
As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!