Pomegranate Aril Preservation and Phenolic Extraction

The dipping of fresh pomegranates was peeled and the arils were separated from the tissues. Then, the arils were washed and treated with a disinfecting solution of calcium hypochlorite (0.25 g/L distilled water) for one minute before being air-dried. The arils are created with a disinfecting solution of calcium hypochlorite (0.25 g/L distilled water) for one minute before being air-dried. The arils were treated as follows: Fresh fruits were (A) extracted pomegranate peels (PPE) 5% (0.1%), (B)ZnONPs 1% (0.02%), (C) ZnONPs 2% (0.04%), (D) ZnONPs 3% (0.06%), (E) ZnONPs 1%/PPE1% (0.02%), (F) ZnONPs 2%/PPE2% (0.04%), (G) ZnONPs 3%/PPE3% (0.06%) Chitosan content in packaged coated fruitsPomegranate peeled cubes were drained after dipping and packaged in plastic trays with approximately 250 g. After that, all boxes were cold stored at 2 °C and 90–95% RH for 20 days and kept in carton boxes. All samples were kept after packaging. Samples were taken at weekly intervals to determine the physical and chemical changes during the storage period. Pomegranate samples were withheld for analysis on a regular basis.
Prepared crude phenolic compound extraction from pomegranate peels(PPE):
The air-dried ground (80 mesh) plant material (20 g for each sample) was extracted with each of the solvents ethanol (ethanol: water, 80:20 v/v) and aqueous methanol (methanol: water, 80:20 v/v) (200 mL) for 6 h at room temperature in an orbital shaker in a water bath in separate experiments. The extracts were separated from the residues by filtering through Whitman No.1 filter paper. The sediments were extracted twice with the same fresh solvent, and the combined extracts were used. Using a rotary evaporator, the combined extracts were concentrated and freed of solvent under reduced pressure at 45 °C. According to [16 (link)]. the dried crude concentrated extracts were weighed to calculate the yield and stored in a refrigerator (−4 °C).