Isolation of cardiac single nuclei was performed as previously described (63 (link)). Briefly, three hearts of 6-week-old mice were extracted in each group. Both ventricles were collected and minced with a razor blade on ice. Minced hearts were homogenized with a dounce grinder. Cell suspension was sequentially filtered through 70- and 40-μm cell strainers (Falcon, 352350 and 352340), and centrifuged at 500g for 5 min. snRNA-seq libraries were generated using Single Cell 3’ Reagent Kits v3 (10x Genomics) according to the manufacturer’s protocol. Sequencing was performed on an Illumina NextSeq 500 system. Sequence data were analyzed by Cell Ranger Single-Cell Software Suite. The cDNA reads were mapped on the mm10/GRCm38 reference genome. DE genes were analyzed (fold change >1.5 in homozygous compared to normal hearts, P value <0.001). Seurat R package (64 (link)) was used for creating GO analysis and uniform manifold approximation and projection (UMAP) analysis.