Phytochemical Analysis of Leaf Constituents

The composition of the leaf constituents was analyzed using a Thermo Finnigan system (Thermo Electron Corporation, USA) tandem with LCQ-Duo ion trap mass spectrometer with an ESI source (ThermoQuest) as described in our previous study [17 (link)]. Separation of constituents was attained using Zorbax Eclipse XDB-C18 (4.6 × 150 mm, 3.5 μm, Agilent, USA). Gradient eluent is composed of water and acetonitrile with 0.1% formic acid in each eluent, from 5 to 30% acetonitrile in 60 min, and flow rate 1 mL/min with a split ratio of 1 : 1 to enter the ESI interface. Xcalibur software (Xcaliburۛ 2.0.7, Thermo Scientific) was used to command the machine [13 (link)].

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Bakrim W.B., Nurcahyanti A.D., Dmirieh M., Mahdi I., Elgamal A.M., El Raey M.A., Wink M, & Sobeh M. (2022). Phytochemical Profiling of the Leaf Extract of Ximenia americana var. caffra and Its Antioxidant, Antibacterial, and Antiaging Activities In Vitro and in Caenorhabditis elegans: A Cosmeceutical and Dermatological Approach. Oxidative Medicine and Cellular Longevity, 2022, 3486257.

Publication 2022

LCQ-Duo ion trap mass spectrometer Zorbax Eclipse XDB-C18 Xcalibur software

Acetonitrile Electron Formic acid Leaf

Corresponding Organization : Université Mohammed VI Polytechnique

Other organizations : Atma Jaya Catholic University of Indonesia, National Research Centre

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Variable analysis

independent variables

Composition of the leaf constituents

dependent variables

Measured outcomes

control variables

Zorbax Eclipse XDB-C18 (4.6 × 150 mm, 3.5 μm, Agilent, USA) for separation of constituents
Water and acetonitrile with 0.1% formic acid as the gradient eluent
Flow rate of 1 mL/min with a split ratio of 1 : 1 to enter the ESI interface
Xcalibur software (Xcaliburۛ 2.0.7, Thermo Scientific) to command the machine

controls

Positive control: Not explicitly mentioned
Negative control: Not explicitly mentioned

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