Multicolor Flow Cytometry Immunophenotyping

Mouse spleen single-cell suspensions and human PBMCs were fluorescently-labeled as previously described (14 (link)). Antibody staining panels used for experiments are available upon request from the corresponding author. Cell viability was determined using live/dead AQUA (Invitrogen). Samples were acquired on a CyAn ADP LX (Beckman Coulter) or sorted with a FACSVantage SE Turbo Sort DIVA v5.0 (Becton Dickinson) and analyzed using FlowJo software version 9.3.3 (Treestar).

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Coquery C.M., Loo W.M., Wade N.S., Bederman A.G., Tung K.S., Lewis J.E., Hess H, & Erickson L.D. (2015). BAFF regulates T follicular helper cells and affects accumulation and IFNγ production in autoimmunity. Arthritis & rheumatology (Hoboken, N.J.), 67(3), 773-784.

Publication 2014

CyAn ADP LX

Antibody Cell Cell viability Human Mouse Spleen

Corresponding Organization :

Other organizations : University of Virginia, Merck (Germany)

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Variable analysis

independent variables

Mouse spleen single-cell suspensions
Human PBMCs

dependent variables

Cell viability

control variables

Antibody staining panels used for experiments

controls

Live/dead AQUA (Invitrogen) for determining cell viability

Annotations

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