In vivo intestinal epithelial permeability was evaluated as previously described [13 (link)]. The rats in each group were fasted for 12 h, and 500 mg/kg fluorescein isothiocyanate (FITC)-dextran solution was intragastrically administered the following day. After 4 h, 4% (w/v) chloral hydrate was intraperitoneally administered. Thereafter, blood samples (5 ml) were collected from the left ventricle, and serum FITC levels determined at 450 nm using a microplate fluorometer from Thermo Fisher Scientific, Inc. (Rochester, NY, USA).
Protocol detail
Intestinal Permeability Evaluation in Rats
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Blood
Chloral hydrate
Fluorescein
Fluorescein isothiocyanate dextran
Intestinal
Isothiocyanate
Left ventricle
Permeability
Rats
Serum
Corresponding Organization :
Other organizations : Affiliated Hospital of Nantong University, Nantong University, Soochow University, Second Affiliated Hospital of Soochow University, Nanjing Medical University
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Variable analysis
independent variables
- Dosage of fluorescein isothiocyanate (FITC)-dextran (500 mg/kg)
- Serum FITC levels
- Fasting duration (12 h)
- Time between FITC-dextran administration and blood collection (4 h)
- Anesthesia (4% (w/v) chloral hydrate administered intraperitoneally)
- No positive or negative controls were explicitly mentioned.
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