Extracting RNA from Mouse Uterine Tissue

Following the manufacturer’s instructions, total RNA was extracted from the mouse uterine tissue by treatment with TRIzol and deoxyribonuclease (Takara Biotechnology, Co., Ltd., Dalian, China). The total RNA concentration was measured as previously described [29 (link)]. cDNA was synthesized using a reverse transcription kit (Takara Biotechnology, Co., Ltd., Dalian, China). Extracted total RNA samples and the synthesized cDNAs were stored at −80 °C.

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Xiong Y., Li W., Lin P., Wang L., Wang N., Chen F., Li X., Wang A, & Jin Y. (2016). Expression and regulation of ATF6α in the mouse uterus during embryo implantation. Reproductive Biology and Endocrinology : RB&E, 14, 65.

Publication 2016

TRIzol deoxyribonuclease reverse transcription kit

Cdnas Deoxyribonuclease Mouse Reverse transcription Tissue Trizol Uterine

Corresponding Organization :

Other organizations : Ministry of Agriculture, Livestock, and Food Supply, Northwest A&F University

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Variable analysis

independent variables

Treatment with TRIzol and deoxyribonuclease (Takara Biotechnology, Co., Ltd., Dalian, China) to extract total RNA from mouse uterine tissue

dependent variables

Total RNA concentration

control variables

Reverse transcription kit (Takara Biotechnology, Co., Ltd., Dalian, China) used to synthesize cDNA
Storage of extracted total RNA samples and synthesized cDNAs at -80 °C

controls

No positive or negative controls were explicitly mentioned in the provided information.

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