Young (3 months; N=12/group) and old (24 months; N=26-29/group; starting group sizes doubled to account for up to 50% attrition) mice were fed a standard chow diet (Envigo) and randomized to receive ad libitum access to either normal drinking water (Control) or drinking water supplemented with 1% (v/v) 3,3-dimethyl-1-butanol (DMB; Sigma-Aldrich) for 8-10 weeks14 (link). Mice were sacrificed at 5-6 (young) or 26-27 months (old) of age. All young mice survived the full intervention and were studied; N=20/group old mice survived and were studied.
Protocol detail
Dietary DMB Supplementation in Mice
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Other organizations : University of Colorado Boulder, Virginia Tech
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Variable analysis
independent variables
- Dietary supplement (normal drinking water vs. drinking water supplemented with 1% (v/v) 3,3-dimethyl-1-butanol (DMB))
- Survival rate of mice
- Mouse age (young: 3 months, old: 24 months)
- Mouse diet (standard chow diet)
- Intervention duration (8-10 weeks)
- Positive control: Not specified
- Negative control: Normal drinking water (Control)
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