In brief 41 TAAs/antigenic fragments (see supporting information), and 2 assay controls (BirA and buffer only) were adsorbed to ELISA plates in duplicate and at 2 concentrations of antigen (100 nM and 50 nM). These proteins were then incubated with serum samples from cancer, high-risk and healthy control cohorts (
Autoantibody Detection via ELISA
In brief 41 TAAs/antigenic fragments (see supporting information), and 2 assay controls (BirA and buffer only) were adsorbed to ELISA plates in duplicate and at 2 concentrations of antigen (100 nM and 50 nM). These proteins were then incubated with serum samples from cancer, high-risk and healthy control cohorts (
Corresponding Organization :
Other organizations : University of Nottingham, Queen's Medical Centre, Astrimmune (United Kingdom), University Hospital Bonn
Variable analysis
- Antigen concentration (100 nM and 50 nM)
- IgG response to the antigens
- BirA control
- Buffer only control
- Incubations with anti-His monoclonal antibody
- Incubations with antigen-specific monoclonal antibodies
Annotations
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