Procedure: In a glass tube, 100µL of the extract at a dose of 1000µg/ml was introduced. Then, 1000µL of 13% picric acid and 1000µL of 4% NaOH were added respectively. The tubes were boiled in a water bath for 10 minutes. In the white tube, the extract was replaced by distilled water. After the tubes were cooled, the optical density was read at 570nm against the blank and the extract concentration was obtained from the glucose calibration curve and expressed as glucose equivalent per mg of dry plant extract.
Protocol detail
Colorimetric Glucose Quantification
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Other organizations : Université de Yaoundé I
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Variable analysis
independent variables
- Extract dose (1000 µg/ml)
- Optical density at 570 nm
- Extract concentration (expressed as glucose equivalent per mg of dry plant extract)
- Volume of extract (100 µL)
- Volume of 13% picric acid (1000 µL)
- Volume of 4% NaOH (1000 µL)
- Time of boiling in water bath (10 minutes)
- Negative control, Distilled water instead of extract
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