Cervical Cancer Cell Lines Co-Cultured with HUVECs

HPV-positive CC cell lines (HeLa cells and Caski cells) and HPV-negative CC cells (C-33A cells and HT-3 cells) were purchased from Shanghai Institute for Biological Sciences (Shanghai, China). Cells were cultured in DMEM (Invitrogen, Carlsbad, CA, USA) containing 10% fetal bovine serum (FBS), 100 U/mL penicillin and 100 mg/mL streptomycin (Sigma-Aldrich, Saint Louis, MO) at 37°C with 5% CO₂.23 (link) Human umbilical vein endothelial cells (HUVECs) were purchased from the cell bank of Wuhan University, and were incubated in a complete medium (EGM BulletKit; Lonza, Walkersville, MD) at 37°C with 5% CO₂. The 0.5–1 × 10¹¹ particles/0.5 × 10⁵ cells CC-MVs were added into the medium to be co-cultured with HUVECs.