Metabolite Profiling by LC-MS

Metabolites were extracted in ice-cold methanol. Endogenous metabolite profiles were obtained using two liquid chromatography-tandem mass spectrometry (LC-MS) methods as described [36 (link)]. Data were acquired using a 4000 QTRAP mass spectrometer (Applied Biosystems/MDS Sciex, Nutley, NJ, USA). Multi-Quant software (Applied Biosystems/MDS Sciex, Nutley, NJ, USA) was used for analysis. Metabolite levels were normalized to protein content [37 (link)].

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Pang B., Zheng X.R., Tian J.X., Gao T.H., Gu G.Y., Zhang R., Fu Y.B., Pang Q., Li X.G, & Liu Q. (2016). EZH2 promotes metabolic reprogramming in glioblastomas through epigenetic repression of EAF2-HIF1α signaling. Oncotarget, 7(29), 45134-45143.

Publication 2016

4000 QTRAP mass spectrometer Multi-Quant software

Cold Liquid chromatography Methanol Protein Tandem mass spectrometry

Corresponding Organization : Shandong University

Other organizations : Qilu Hospital of Shandong University, Shandong Provincial Hospital, Jinan Central Hospital

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Variable analysis

independent variables

Independent variables not explicitly mentioned.

dependent variables

Endogenous metabolite profiles

control variables

Protein content [37 (link)]

controls

Positive control: Not mentioned.
Negative control: Not mentioned.

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